Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prokineticin Receptor 2 (extracellular) Blocking Peptide (#BLP-PR042) is the original antigen used for immunization during Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042) generation. The blocking peptide binds and ‘blocks’ Anti-Prokineticin Receptor 2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (1:200).
2. Anti-Prokineticin Receptor 2 (extracellular) Antibody, preincubated with Prokineticin Receptor 2 (extracellular) Blocking Peptide (#BLP-PR042).
Expression of Prokineticin receptor 2 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in small, medium and large DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
- (C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat PROKR2 (Accession Q8R415).
Accession (Uniprot) Number Q8R415
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
