Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064) is the original antigen used for immunization during Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064) generation. The blocking peptide binds and ‘blocks’ Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), rat stomach (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).
4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6):1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).
4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody, preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
Expression of Prostanoid EP2 receptor in rat cerebellumImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:100). Prostanoid EP2 receptor staining (red) is detected in Purkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.
Properties
Sequence
- (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928).
Accession (Uniprot) Number Q62928
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
