Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065) is the original antigen used for immunization during Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065) generation. The blocking peptide binds and ‘blocks’ Anti-Prostaglandin E Receptor EP3/PTGER3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney (lanes 1 and 3) and pancreas (lanes 2 and 4) membranes:1,2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:200).
3,4. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
Western blot analysis of mouse kidney membranes:1. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:200).
2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
Western blot analysis of human Jurkat T-cell leukemia cell lysate:1. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:400).
2. Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody, preincubated with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065).
Expression of Prostaglandin E Receptor EP3 in mouse parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Prostaglandin E Receptor EP3 (PTGER3) Antibody (#APR-065), (1:1000), followed by goat anti-rabbit-Alexa-488. A. EP3 Receptor immunoreactivity (green) appears in neuronal processes in the parietal cortex (arrows). B. Pre-incubation of the antibody with Prostaglandin E Receptor EP3/PTGER3 Blocking Peptide (#BLP-PR065), suppressed staining. Cell nuclei are stained with DAPI (blue). L1-2 = cortical layers 1 and 2, L3 = cortical layer 3.
Properties
Sequence
- (C)RAPHWYASHMKTR, corresponding to amino acid residues 137-149 of mouse prostanoid EP3 receptor (Accession P34980).
Accession (Uniprot) Number P34980
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody
Standard Quality Control Of Each Lot Western blot analysis.
