Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066) is the original antigen used for immunization during Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066) generation. The blocking peptide binds and ‘blocks’ Anti-Prostaglandin E Receptor EP4/PTGER4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):1,2. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066), (1:200).
3,4. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody, preincubated with Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):1,2. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066), (1:400).
3,4. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody, preincubated with Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066).
Properties
Sequence
- (C)RKTVLSKAIEKIK, corresponding to amino acid residues 336-348 of rat prostanoid EP4 receptor (Accession P43114).
Accession (Uniprot) Number P43114
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
