Overview
Type: Synthetic peptide
Form: Lyophilized powder
Prostaglandin F2-α Receptor/PTGFR Blocking Peptide (#BLP-PR067) is the original antigen used for immunization during Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) generation. The blocking peptide binds and ‘blocks’ Anti-Prostaglandin F2-α Receptor/PTGFR primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell line (lanes 3 and 6) lysates:1-3. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067), (1:200).
4-6. Anti-Prostaglandin F2-α Receptor/PTGFR Antibody, preincubated with Prostaglandin F2-α Receptor/PTGFR Blocking Peptide (#BLP-PR067).
Expression of Prostaglandin F2-alpha receptor in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Prostaglandin F2-α Receptor/PTGFR Antibody (#APR-067) (1:200), followed by goat anti-rabbit-AlexaFluor-488. PTGFR staining (green) in mouse hippocampal dentate gyrus is detected in cell bodies (arrows). Cell nuclei are stained with DAPI (blue). Mouse brain hippocampal dentate gyrus sections were obtained two hours following kainate acid intraventricular injection.
Properties
Sequence
- (C)RSQQHRQGRSHHLE, corresponding to amino acid residues 233 - 246 of rat Prostaglandin F2-α Receptor (Accession P43118).
Accession (Uniprot) Number P43118
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
