Overview
Type: Synthetic peptide
Form: Lyophilized powder
PSD-95 Blocking Peptide (#BLP-PZ009) is the original antigen used for immunization during Anti-PSD-95 Antibody (#APZ-009) generation. The blocking peptide binds and ‘blocks’ Anti-PSD-95 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-PSD-95 Antibody (#APZ-009), (1:200).
3,4. Anti-PSD-95 Antibody, preincubated with PSD-95 Blocking Peptide (#BLP-PZ009).
Expression of PSD-95 in mouse parietal cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PSD-95 Antibody (#APZ-009), (1:400), followed by anti-rabbit-Cy3 antibody (red). Staining appears mainly in the pyramidal cell body (arrows). Nuclei were stained with DAPI (blue).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1-2. Guinea Pig Anti-PSD-95 Antibody (#APZ-009-GP), (1:200).
3-4. Guinea Pig Anti-PSD-95 Antibody, preincubated with PSD-95 Blocking Peptide (BLP-PZ009).
Properties
Sequence
- (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95 (Accession P78352).
Accession (Uniprot) Number P78352
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
