Overview
Type: Synthetic peptide
Form: Lyophilized powder
PTH2R (extracellular) Blocking Peptide (#BLP-PR052) is the original antigen used for immunization during Anti-PTH2R (extracellular) Antibody (#APR-052) generation. The blocking peptide binds and ‘blocks’ Anti-PTH2R (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-PTH2R (extracellular) Antibody (#APR-052), (1:600).
3,4. Anti-PTH2R (extracellular) Antibody, preincubated with PTH2R (extracellular) Blocking Peptide (#BLP-PR052).
Properties
Sequence
- CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat PTH2R (Accession P70555).
Accession (Uniprot) Number P70555
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
