Overview
Type: Synthetic peptide
Form: Lyophilized powder
QRFPR/GPR103 (extracellular) Blocking Peptide (#BLP-GR033) is the original antigen used for immunization during Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033) generation. The blocking peptide binds and ‘blocks’ Anti-QRFPR/GPR103 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3), mouse brain membranes (lanes 2 and 4) and human LNCaP cell lysate (lanes 3 and 6):1-3. Anti-QRFPR/GPR103 (extracellular) Antibody (#AGR-033), (1:500).
4-6. Anti-QRFPR/GPR103 (extracellular) Antibody, preincubated with QRFPR/GPR103 (extracellular) Blocking Peptide (#BLP-GR033).
Properties
Sequence
- (C)HNLTREQFIHRYGLR, corresponding to amino acid residues 18-32 of rat QRFP receptor (Accession P83858).
Accession (Uniprot) Number P83858
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
