Overview
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified from non-immunized rabbits.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480-515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc, ih
Negative control in immunohistochemistry and surface staining flow cytometry applications.
Direct flow cytometry
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), alongside cell surface detection of P2RX7 by direct flow cytometry in a live intact human THP-1 monocytic leukemia cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), (2.5µg).
___ Cells + Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG), (2.5µg).
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG) alongside cell surface detection of CXCR4 by direct flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), (2.5µg).
___ Cells + Anti-CXCR4 (extracellular)-ATTO Fluor-488 Antibody (#ACR-014-AG), (2.5µg).
Lyophilized Powder
For research purposes only, not for human use
Last Update: 25/10/2024
Specifications
Citations
Citations
