Overview
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified from non-immunized rabbits.
Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540-565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc, ih
Negative control in immunohistochemistry and surface staining flow cytometry applications.
Immunohistochemistry
Comparison of immunostaining with Anti-KV1.5 antibody to immunostaining with isotype control in rat cerebellumA. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody (#APC-004-AO), (1:80). KV1.5 immunoreactivity (orange), appears at Bergmann glial cells (horizontal arrow) and their dendrites (vertical arrows). B. Staining of sequential sections with Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (1:80), shows background signal. Cell nuclei are stained with DAPI (blue).
Comparison of immunostaining with Anti-p75 NGF Receptor antibody to immunostaining with isotype control in mouse nucleus basalis magnocelullaris (NBM).A. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-550 Antibody (#ANT-007-AO), (1:80). p75 immunoreactivity (orange), appears in NBM cells (arrows). B. Staining of sequential sections with Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (1:80), shows background signal. Cell nuclei are stained with DAPI (blue).
Direct flow cytometry
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), alongside cell surface detection of AT1R by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (5µg).
___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO), (5µg).
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO) alongside cell surface detection of STIM1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (5µg).
___ Cells + Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO), (5µg).
Lyophilized Powder
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-550 fluorophore.
For research purposes only, not for human use
Last Update: 20/10/2024
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