Overview
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified from non-immunized rabbits.
Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540-565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc, ih
Negative control in immunohistochemistry and surface staining flow cytometry applications.
Direct flow cytometry
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), alongside cell surface detection of AT1R by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (5µg).
___ Cells + Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO), (5µg).
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO) alongside cell surface detection of STIM1 by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), (5µg).
___ Cells + Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO), (5µg).
Lyophilized Powder
For research purposes only, not for human use
Last Update: 20/10/2024
Specifications
Citations
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