Overview
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified from non-immunized rabbits.
Label ATTO-647N. Maximum absorption 646 nm; maximum fluorescence 664 nm. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm. It can be used in flow cytometry (FACS) using the red (637 nm) laser.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc, ih
Negative control in immunohistochemistry and surface staining flow cytometry applications.
Immunohistochemistry
Comparison of immunostaining with Anti-GFAP antibody to immunostaining with isotype control in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GFAP-ATTO Fluor-647N Antibody (#AFP-001-FRN), (1:80). A. Staining in the hippocampal dentate gyrus region, shows GFAP immunoreactivity (purple) in astrocytes (arrows). B. Staining of sequential sections with Rabbit IgG Isotype Control-ATTO Fluor-647N (#RIC-001-FRN), (1:80), shows background signal. Cell nuclei are stained with DAPI (blue). H = hilus, G = granule layer, OML = outer molecular layer.
Direct flow cytometry
Establishing the effectiveness of the Rabbit IgG Isotype Control-ATTO Fluor-647N (#RIC-001-FRN), alongside cell surface detection of KCNQ1 (extracellular) by direct flow cytometry in a live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:___ Cells.
___ Cells + Rabbit IgG Isotype Control-ATTO Fluor-647N (#RIC-001-FRN), (1µg).
___ Cells + Anti-KCNQ1 (extracellular)-ATTO Fluor-647N Antibody (#APC-168-FRN), (1µg).
Lyophilized Powder
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-647N (#RIC-001-FRN), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-647N fluorophore.
For research purposes only, not for human use
Last Update: 29/10/2024
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