Overview
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Purity Serum from non-immunized rabbits was pooled and the IgG fraction was purified on a Protein A column.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The IgG fraction was purified from non-immunized rabbits.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Working dilution The isotype control antibody should be used at the exact same dilution of the experimental antibody
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Flow cytometry
Applications: fc, ih, ip
Negative control in immunoprecipitation, immunohistochemistry and surface staining flow cytometry applications.
Indirect flow cytometry
Establishing the effectiveness of the Rabbit IgG Isotype Control, alongside cell surface detection of NK1R by indirect flow cytometry in live intact mouse J774 macrophage cell line. (A) In the absence of an Fc block. (B) In the presence of an Fc block:___ Cells.
___ Cells + goat-anti-rabbit-PE.
___ Cells + Rabbit IgG Isotype Control (#RIC-001), (2.5µg) + goat-anti-rabbit-PE.
___ Cells + Anti-Neurokinin 1 Receptor (NK1R) (extracellular) Antibody (#ATR-001), (2.5µg) + goat-anti-rabbit-PE.
Establishing the effectiveness of the Rabbit IgG Isotype Control, alongside cell surface detection of CXCR4 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Rabbit IgG Isotype Control (#RIC-001), (2.5µg) + goat-anti-rabbit-FITC.
___ Cells + Anti-CXCR4 (extracellular) Antibody (#ACR-014), (2.5µg) + goat-anti-rabbit-FITC.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 30/10/2024
Specifications
Citations
Citations
