Overview
Type: Synthetic peptide
Form: Lyophilized Powder
RAGE (extracellular) Blocking Peptide (#BLP-NR176) is the original antigen used for immunization during Anti-RAGE (extracellular) Antibody (#ANR-176) generation. The blocking peptide binds and ‘blocks’ Anti-RAGE (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):1, 2. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
3, 4. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):1-4. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
5-8. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
Properties
Sequence
- Peptide (C)GKETKSNYRVRVYQIPGK, corresponding to amino acid residues 106 - 123 of human RAGE (Accession Q15109).
Accession (Uniprot) Number Q15109
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
