Overview
Type: Synthetic peptide
Form: Lyophilized powder
RAMP1 (extracellular) Blocking Peptide (#BLP-RR021) is the original antigen used for immunization during Anti-RAMP1 (extracellular) Antibody (#ARR-021) generation. The blocking peptide binds and ‘blocks’ Anti-RAMP1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat pancreas (lanes 3 and 6):1-3. Anti-RAMP1 (extracellular) Antibody (#ARR-021).
4-6. Anti-RAMP1 (extracellular) Antibody, preincubated with RAMP1 (extracellular) Blocking Peptide (#BLP-RR021).
The antibody was used at a concentration of 1:400 for brain lysates and 1:200 for pancreas lysates.
Expression of RAMP1 in rat brainImmunohistochemical staining of rat free-floating frozen section using Anti-RAMP1 (extracellular) Antibody (#ARR-021). A. In the parietal cortex, RAMP1 immunoreactivity (green) appears in pyramidal neurons (arrows). B. In the hippocampus, RAMP1 staining (green) is detected in the CA1 region (arrows). Nuclei are demonstrated using DAPI as the counterstain (blue).
Properties
Sequence
- CRDPDYGTLIQE, corresponding to amino acid residues 27-38 of rat RAMP1 (Accession Q9JJ74).
Accession (Uniprot) Number Q9JJ74
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
