Overview
Type: Synthetic peptide
Form: Lyophilized powder
RAMP2 (extracellular) Blocking Peptide (#BLP-RR022) is the original antigen used for immunization during Anti-RAMP2 (extracellular) Antibody (#ARR-022) generation. The blocking peptide binds and ‘blocks’ Anti-RAMP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat lung (lanes 1 and 4, 1:200), mouse heart (lanes 2 and 5, 1:200) and rat aortic endothelial cell (RAEC) (lanes 3 and 6, 1:400) lysates:1-3. Anti-RAMP2 (extracellular) Antibody (#ARR-022).
4-6. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).
Western blot analysis of rat spleen (lanes 1 and 3) and cortex (lanes 2 and 4):1,2. Anti-RAMP2 (extracellular) Antibody (#ARR-022), (1:200).
3,4. Anti-RAMP2 (extracellular) Antibody, preincubated with RAMP2 (extracellular) Blocking Peptide (#BLP-RR022).
Properties
Sequence
- (C)HPTEDSLLSKGKMED, corresponding to amino acid residues 54-68 of rat RAMP2 (Accession Q9JHJ1).
Accession (Uniprot) Number Q9JHJ1
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
