Overview
Type: Synthetic peptide
Form: Lyophilized powder
Rat CMKLR1 (extracellular) Blocking Peptide (#BLP-CR033) is the original antigen used for immunization during Anti-Rat CMKLR1 (extracellular) Antibody (#ACR-033) generation. The blocking peptide binds and ‘blocks’ Anti-Rat CMKLR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cerebellum lysate (lanes 1 and 3) and rat heart membrane (lanes 2 and 4):1,2. Anti-Rat CMKLR1 (extracellular) antibody (1:200), (ACR-033).
3,4. Anti-Rat CMKLR1 (extracellular) antibody, preincubated with Rat CMKLR1 (extracellular) Blocking Peptide (#BLP-CR033).
Expression of CMKLR1 in rat supraoptic nucleus (SON).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Rat CMKLR1 (extracellular) Antibody (#ACR-033), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CMKLR1 immunoreactivity (green) appears in neurons of the SON (arrows). B. Pre-incubation of the antibody with Rat CMKLR1 (extracellular) Blocking Peptide (#BLP-CR033), suppressed staining. Cell nuclei are stained with DAPI (blue). OT = optic tract.
Expression CMKLR1 in rat paraventricular nucleus (PVN).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Rat CMKLR1 (extracellular) Antibody (#ACR-033), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. CMKLR1 immunoreactivity (green) appears along the wall of the third ventricle (arrows). B. Pre-incubation of the antibody with Rat CMKLR1 (extracellular) Blocking Peptide (#BLP-CR033), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
Properties
Sequence
- (C)HSTHPRTDPVGYSRH, corresponding to amino acid residues 201-215 of rat Chemerin receptor 1 (Accession O35786).
Accession (Uniprot) Number O35786
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
