Overview
Type: Synthetic peptide
Form: Lyophilized powder
TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029) is the original antigen used for immunization during Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV1/VR1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of TRPV1 in rat DRG:1. Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
2. Anti-TRPV1 (VR1) (extracellular) Antibody, preincubated with TRPV1/VR1 (extracellular) Blocking Peptide (#BLP-CC029).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 4), mouse TK-1 T-cell lymphoma cell line lysate (lanes 2 and 5) and mouse P815 mastocytoma cell line lysate (lanes 3 and 6):1-3. Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:200).
4-6. Anti-TRPV1 (VR1) (extracellular) Antibody, preincubated with TRPV1 (VR1) (extracellular) Blocking Peptide (#BLP-CC029).
Expression of TRPV1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV1 (VR1) (extracellular) Antibody (#ACC-029), (1:100) followed by goat anti-rabbit-ATTO Fluor-594 secondary antibody (red). TRPV1 is expressed in medium and small DRG neurons. Hoechst 33342 is used as the counterstain (blue).
Properties
Sequence
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Peptide (C)NSLPMESTPHK(S)RGS, corresponding to amino acid residues 605-619 of rat TRPV1 (Accession O35433).
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Accession (Uniprot) Number O35433
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
