Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014) is the original antigen used for immunization during Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014) generation. The blocking peptide binds and ‘blocks’ Anti-Renin Receptor/ATP6AP2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):1, 2. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
3, 4. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Expression of Renin Receptor in rat cerebellum.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Renin Receptor immunoreactivity (green) appears in neuronal profiles in the purkinje cell layer (arrows). B. Pre-incubation of the antibody with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (BLP-AR014), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor (Accession Q9CYN9).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
