Overview
Alternative Name Reciniferatoxin, RTX
Lyophilized Powder yes
Origin Euphorbia resinifera (Moroccan cactus).
Source Natural
MW: 628.72
Purity: 95-98% (HPLC)
Effective concentration 20 nM - 1 µM.
Structure
Chemical name 4-Hydroxy-3-methoxy-[(2S,3aR,3bS,6aR,9aR,9bR,10R,11aR)- 3a,3b,6,6a,9a,10,11,11aoctahydro-6a-hydroxy-8,10-dimethyl-11a-(1-methylethenyl)-7-oxo-2-(phenylmethyl)-7H-2,9 b-epoxyazuleno[5,4-e]-1,3-benzodioxol-5-yl]benzeneaceta.
Molecular formula C37H40O9.
CAS No.: 57444-62-9
Activity Resiniferatoxin is a potent and selective activator of the TRPV1 (Vanilloid/Capsaicin receptor 1) channel1.
References-Activity
- Szallasi, A. and Blumberg, P.M. (1989) Neuroscience 30, 515.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility Methanol or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two week at 4°C or six months at -20°C.
Our bioassay
Alomone Labs Resiniferatoxin activates TRPV1 receptors expressed in Xenopus oocytes.Time course of TRPV1 currents, irreversibly activated by 100nM Resiniferatoxin (#R-400) while membrane potential was held at -60 mV.
Alomone Labs Resiniferatoxin induces Ca2+ influx via activation of TRPV1 expressed in HEK293 cells.Cells were loaded with 1mM fluo-3 AM and then stimulated with Resiniferatoxin (#R-400). The left panel shows the intracellular Ca2+ levels 10 sec post stimulation with different concentrations of Resiniferatoxin plotted against drug concentration (ED50 = 2 µM) . The right panel shows cytoplasmic Ca2+ before and after stimulation of cells with 10 µM Resiniferatoxin.
Scientific background
Resiniferatoxin is a potent and selective agonist of the TRPV1 (Vanilloid/Capsaicin receptor) channel.1 Application of sub-nanomolar concentrations of Resiniferatoxin irreversibly induces a sustained slow current and depolarization in DRG neurons.2
Treatment of rats with Resiniferatoxin, like treatment with capsaicin, causes hypothermia, neurogenic inflammation and pain. These responses were followed by a loss of thermoregulation, by desensitization to neurogenic inflammation and by chemical and thermal analgesia, with a cross-tolerance between Resiniferatoxin and capsaicin.1
Target TRPV1 channels
Lyophilized Powder
For research purposes only, not for human use
Last Update: 06/06/2024
Specifications
Citations
Citations
