Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ret (extracellular) Blocking Peptide (#BLP-NT025) is the original antigen used for immunization during Anti-Ret (extracellular) Antibody (#ANT-025) generation. The blocking peptide binds and ‘blocks’ Anti-Ret (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Ret (extracellular) Antibody (#ANT-025), (1:200).
3,4. Anti-Ret (extracellular) Antibody, preincubated with Ret (extracellular) Blocking Peptide (#BLP-NT025).
Expression of Ret in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Ret (extracellular) Antibody (#ANT-025), (1:50). Ret staining (red) is detected in DRG neurons. Hoechst 33342 (blue) is used to stain cell nuclei.
Properties
Sequence
- CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret (Accession P07949).
Accession (Uniprot) Number P07949
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
