Overview
Type: Synthetic peptide
Form: Lyophilized Powder
RGMA (extracellular) Blocking Peptide (#BLP-NR126) is the original antigen used for immunization during Anti-RGMA (extracellular) Antibody (#ANR-126) generation. The blocking peptide binds and ‘blocks’ Anti-RGMA (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):1-2. Anti-RGMA (extracellular) Antibody (#ANR-126), (1:500).
3-4. Anti-RGMA (extracellular) Antibody, preincubated with RGMA (extracellular) Blocking Peptide (BLP-NR126).
Western blot analysis of human glioblastoma U-87 MG cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):1-2. Anti-RGMA (extracellular) Antibody (#ANR-126), (1:500).
3-4. Anti-RGMA (extracellular) Antibody, preincubated with RGMA (extracellular) Blocking Peptide (BLP-NR126).
Expression of RGMA in mouse hippocampus:Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RGMA (extracellular) Antibody (#ANR-126), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed RGMA immunoreactivity (green) in neuronal outlines (arrows) in the hilus. B. Pre-incubation of the antibody with RGMA (extracellular) Blocking Peptide (BLP-NR126), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of RGMA in mouse cerebellum:Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RGMA (extracellular) Antibody (#ANR-126), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. RGMA immunoreactivity (green) appears in purkinje cells (arrows) and their dendrites in the molecular layer (MOL). B. Pre-incubation of the antibody with RGMA (extracellular) Blocking Peptide (BLP-NR126), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)ERSDSPEI(S)HYEKSFHK, corresponding to amino acid residues 138 - 154 of mouse RGMA (Accession Q6PCX7). Extracellular, N-terminus (pro-domain).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
