Overview
Type: Synthetic peptide
Form: Lyophilized powder
RIC3 Blocking Peptide (#BLP-NC020) is the original antigen used for immunization during Anti-RIC3 Antibody (#ANC-020) generation. The blocking peptide binds and ‘blocks’ Anti-RIC3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-RIC3 Antibody (#ANC-020), (1:200).
2. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):1-3. Anti-RIC3 Antibody (#ANC-020), (1:200).
4-6. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Expression of RIC-3 in mouse olfactory bulb and fornixImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-RIC3 Antibody (#ANC-020), (1:400), followed by goat-anti-rabbit-AlexaFluor-488. A. RIC-3 staining in mouse olfactory bulb shows staining in the external plexiform layer (EPL) and in some glomeruli (G). Arrows point at glomeruli with RIC-3 positive core. B. RIC-3 staining in mouse fornix is detected in neuronal profiles (vertical arrows) and neuronal processes (horizontal arrow). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3 (Accession Q8BPM6).
Accession (Uniprot) Number Q8BPM6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
