Cat #: BLP-NC020
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3 (Accession Q8BPM6).
Accession (Uniprot) Number Q8BPM6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-RIC3 Antibody (#ANC-020), (1:200).
2. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020). - Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):1-3. Anti-RIC3 Antibody (#ANC-020), (1:200).
4-6. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020). - Mouse brain sections (1:400).
Last update: 02/01/2024