Overview
Type: Synthetic peptide
Form: Lyophilized powder
Robo1 (extracellular) Blocking Peptide (#BLP-NR181) is the original antigen used for immunization during Anti-Robo1 (extracellular) Antibody (#ANR-181) generation. The blocking peptide binds and ‘blocks’ Anti-Robo1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1, 2. Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200).
3, 4. Anti-Robo1 (extracellular) Antibody, preincubated with Robo1 (extracellular) Blocking Peptide (#BLP-NR181) (#BLP-NR181).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 5) and human THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200).
4-6. Anti-Robo1 (extracellular) Antibody, preincubated with Robo1 (extracellular) Blocking Peptide (#BLP-NR181) (#BLP-NR181).
Expression of Robo1 in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Robo1 (extracellular) Antibody (#ANR-181), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Robo1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with Robo1 (extracellular) Blocking Peptide (#BLP-NR181), suppresses staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number Q9Y6N7
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
