Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)RPAEDPWKGQRGLAE, corresponding to amino acid residues 656 - 670 of mouse Robo3 (Accession Q9Z2I4).
Accession (Uniprot) Number Q9Z2I4
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-Robo3 (extracellular) Antibody (#ANR-183), (1:200).
3-4. Anti-Robo3 (extracellular) Antibody, preincubated with Robo3 (extracellular) Blocking Peptide (BLP-NR183).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysates:1. Anti-Robo3 (extracellular) Antibody (#ANR-183), (1:200).
2. Anti-Robo3 (extracellular) Antibody, preincubated with Robo3 (extracellular) Blocking Peptide (BLP-NR183).
Last update: 02/01/2024
