Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ryanodine Receptor 1 Blocking Peptide (#BLP-RR001) is the original antigen used for immunization during Anti-Ryanodine Receptor 1 Antibody (#ARR-001) generation. The blocking peptide binds and ‘blocks’ Anti-Ryanodine Receptor 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat skeletal muscle lysates:1. Anti-Ryanodine Receptor 1 Antibody (#ARR-001), (1:200).
2. Anti-Ryanodine Receptor 1 Antibody, preincubated with Ryanodine Receptor 1 Blocking Peptide (#BLP-RR001).
Expression of RyR1 in rat skeletal muscleImmunohistochemical staining of paraffin embedded sections of rat quadriceps using Anti-Ryanodine Receptor 1 Antibody (#ARR-001), (1:100). RyR1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue. Hematoxilin is used as the counterstain.
Expression of RyR1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections using Anti-Ryanodine Receptor 1 Antibody (#ARR-001), (1:100), (green). A. The highest expression of RyR1 is in the molecular layer (asterisk). B. In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C. Merged image of panels A and B demonstrates that RyR1 is localized surrounding the dendritic tree of Purkinje cells. DAPI is used as the counterstain.
Properties
Sequence
- (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1 (Accession P21817).
Accession (Uniprot) Number P21817
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
