Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ryanodine Receptor 2 Blocking Peptide (#BLP-RR002) is the original antigen used for immunization during Anti-Ryanodine Receptor 2 Antibody (#ARR-002) generation. The blocking peptide binds and ‘blocks’ Anti-Ryanodine Receptor 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes:1. Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:200).
2. Anti-Ryanodine Receptor 2 Antibody, preincubated with Ryanodine Receptor 2 Blocking Peptide (#BLP-RR002).
Expression of RyR2 in rat cardiac muscleImmunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
Expression of RyR2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 Antibody (#ARR-002), (1:100), (green fluorescence). A. The highest expression of RyR2 is in the molecular layer (asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that RyR2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
Properties
Sequence
- CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2 (Accession Q92736).
Accession (Uniprot) Number Q92736
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
