Overview
Type: Synthetic peptide
Form: Lyophilized powder
Ryanodine Receptor 3 Blocking Peptide (#BLP-RR003) is the original antigen used for immunization during Anti-Ryanodine Receptor 3 Antibody (#ARR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Ryanodine Receptor 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (1:200).
2. Anti-Ryanodine Receptor 3 Antibody, preincubated with Ryanodine Receptor 3 Blocking Peptide (#BLP-RR003).
Expression of RyR3 in rat cortexImmunohistochemical staining of rat brain frozen sections with Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (red), (1:100). RyR3 is widely expressed in neurons of the cortex. Hoechst 33342 is used as the counterstain.
Properties
Sequence
- CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3 (Accession Q15413).
Accession (Uniprot) Number Q15413
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
