Overview
Type: Synthetic peptide
Form: Lyophilized powder
S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011) is the original antigen used for immunization during Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR1/EDG1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of (RAEC) rat aortic endothelial cell line lysates (lanes 1 and 3) and A-10 Rat thoracic aorta smooth muscle (lane 2 and 4) cell lysates:1,2. Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:200).
3,4. Anti-S1PR1 (EDG1) (extracellular) Antibody, preincubated with S1PR1/EDG1 (extracellular) Blocking Peptide (#BLP-SR011).
Expression of Sphingosine 1-phosphate receptor 1 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human S1PR1 (Accession P21453).
Accession (Uniprot) Number P21453
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
