Cat #: BLP-SR012
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2 (Accession P52592).
Accession (Uniprot) Number P52592
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).
Last update: 02/01/2024