Overview
Type: Synthetic peptide
Form: Lyophilized powder
S1PR3/EDG3 (extracellular) Blocking Peptide (#BLP-SR013) is the original antigen used for immunization during Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR3/EDG3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart (lanes 1 and 3), rat kidney (lanes 2 and 4), human Jurkat acute T cell leukemia (lanes 5 and 7) and mouse WEHI B cell lymphoma (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013), (1:200).
3,4,7,8. Anti-S1PR3 (EDG3) (extracellular) Antibody, preincubated with S1PR3/EDG3 (extracellular) Blocking Peptide (#BLP-SR013).
Properties
Sequence
- (C)DYVGKLAGRLRD, corresponding to amino acid residues 23-34 of mouse S1PR3 (Accession Q9Z0U9).
Accession (Uniprot) Number Q9Z0U9
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
