Overview
Type: Synthetic peptide
Form: Lyophilized powder
S1PR5/EDG8 (extracellular) Blocking Peptide (#BLP-SR015) is the original antigen used for immunization during Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015) generation. The blocking peptide binds and ‘blocks’ Anti-S1PR5/EDG8 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat cortex (lanes 1 and 5), rat cerebellum (lanes 2 and 6), rat hippocampus (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:1-4. Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015), (1:200).
5-8. Anti-S1PR5/EDG8 (extracellular) Antibody, preincubated with S1PR5/EDG8 (extracellular) Blocking Peptide (#BLP-SR015).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human NK-92 natural killer cell line lysate (lanes 2 and 5) and human HL-60 promyelocytic leukemia cell line lysate (lanes 3 and 6) lysates:1-3. Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015), (1:200).
4-6. Anti-S1PR5/EDG8 (extracellular) Antibody, preincubated with the negative control antigen.
Properties
Sequence
- (C)KLRGARYQPGAGLRAD, corresponding to amino acid residues 24-39 of mouse S1PR5 (EDG8) (Accession Q91X56).
Accession (Uniprot) Number Q91X56
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
