Overview
Type: Synthetic peptide
Form: Lyophilized powder
SAP-97/DLG1 Blocking Peptide (#BLP-PZ010) is the original antigen used for immunization during Anti-SAP-97/DLG1 Antibody (#APZ-010) generation. The blocking peptide binds and ‘blocks’ Anti-SAP-97/DLG1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6):1-3. Anti-SAP-97/DLG1 Antibody (#APZ-010), (1:200).
4-6. Anti-SAP-97/DLG1 Antibody, preincubated with SAP-97/DLG1 Blocking Peptide (#BLP-PZ010).
Properties
Sequence
- (C)EYRSKLSQTEDRQLR, corresponding to amino acid residues 17-31 of human SAP-97 (Accession Q12959).
Accession (Uniprot) Number Q12959
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
