Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017) is the original antigen used for immunization during Anti-SCN11A (NaV1.9) Antibody (#ASC-017) generation. The blocking peptide binds and ‘blocks’ Anti-SCN11A/Nav1.9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysates:1. Anti-SCN11A (NaV1.9) Antibody (#ASC-017), (1:200).
2. Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
Expression of NaV1.9 in rat embryo DRGImmunohistochemical staining of formalin frozen rat embryo dorsal root ganglion (DRG) sections with Anti-SCN11A (NaV1.9) Antibody (#ASC-017). A. Cells within the DRG were stained (see solid line frame enlarged in B) as well as fibers and the area of entry of dorsal root into the spinal cord (see dashed line frame enlarged in C). DAPI is used as the counterstain.- Human trigeminal ganglia and dental pulp (1:100) (Wells, J.E. et al. (2007) J. Endodontics 33, 1172.).
Western blot analysis of rat brain membrane:1. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP), (1:200).
2. Guinea pig Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).
Expression of NaV1.9 in rat DRGImmunohistochemical staining of NaV1.9 in rat dorsal root ganglion (DRG) using Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP). A. NaV1.9 staining (green) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counter stain (blue). C. Merged image of A and B.
Properties
Sequence
- CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457).
Accession (Uniprot) Number O88457
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
