Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- CNGDLSSLDVAKVKVHND, corresponding to amino acid residues 1748-1765 of rat NaV1.9 (Accession O88457).
Accession (Uniprot) Number O88457
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat DRG lysates:1. Anti-SCN11A (NaV1.9) Antibody (#ASC-017), (1:200).
2. Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).- Rat embryo DRG formalin frozen sections. Human trigeminal ganglia and dental pulp (1:100) (see Wells, J.E. et al. (2007) J. Endodontics 33, 1172.).
Western blot analysis of rat brain membrane:1. Guinea pig Anti-SCN11A (NaV1.9) Antibody (#ASC-017-GP), (1:200).
2. Guinea pig Anti-SCN11A (NaV1.9) Antibody, preincubated with SCN11A/Nav1.9 Blocking Peptide (#BLP-SC017).- Rat dorsal root ganglion (DRG). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
