Overview
Type: Synthetic peptide
Form: Lyophilized Powder
SCN1A (NaV1.1) (extracellular) Blocking Peptide (#BLP-SC051) is the original antigen used for immunization during Anti-SCN1A (NaV1.1) (extracellular) Antibody (#ASC-051) generation. The blocking peptide binds and ‘blocks’ Anti-SCN1A (NaV1.1) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-SCN1A (NaV1.1) (extracellular) Antibody (#ASC-051), (1:200).
3-4. Anti-SCN1A (NaV1.1) (extracellular) Antibody, preincubated with SCN1A (NaV1.1) (extracellular) Blocking Peptide (BLP-SC051).
Expression of Nav1.1 in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SCN1A (NaV1.1) (extracellular) Antibody (#ASC-051), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Nav1.1 immunoreactivity (green) appears in cerebellar Purkinje cells (vertical arrows) and their dendrites (horizontal arrows). B. Pre-incubation of the antibody with SCN1A (NaV1.1) (extracellular) Blocking Peptide (BLP-SC051), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, MOL = molecular layer.
Expression of Nav1.1 in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-SCN1A (NaV1.1) (extracellular) Antibody (#ASC-051), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Nav1.1 immunoreactivity (green) appears in the cell body of cortical pyramidal neurons (horizontal arrows) and in their dendrites (vertical arrows). B. Pre-incubation of the antibody with SCN1A (NaV1.1) (extracellular) Blocking Peptide (BLP-SC051), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)EFDWKSYIQDSRYH, corresponding to amino acid residues 311 - 324 of mouse SCN1A (Accession A2APX8). Extracellular, 3rd loop.
Accession (Uniprot) Number A2APX8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
