Name: SCN1A/Nav1.1 Blocking Peptide
Brand: Alomone Labs
SKU: BLP-SC001

Overview

Cat #: BLP-SC001

Type: Synthetic peptide

Form: Lyophilized powder

SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001) is the original antigen used for immunization during Anti-SCN1A (Na_V1.1) Antibody (#ASC-001) generation. The blocking peptide binds and ‘blocks’ Anti-SCN1A/Nav1.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Certificate of Analysis SDS

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain membranes:
1. Anti-SCN1A (Na_V1.1) Antibody (#ASC-001), (1:200).
2. Anti-SCN1A (Na_V1.1) Antibody, preincubated with SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001).
Expression of Na_V1.1 sodium channel in mouse cerebellum
Immunohistochemical staining of mouse cerebellum using Anti-SCN1A (Na_V1.1) Antibody (#ASC-001). A. The distribution of Na_V1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of Na_V1.1 and Parvalbumin.
Human brain (1:400) (Wang, W. et al. (2011) Brain Res. 1389, 61.).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:
1,2. Guinea pig Anti-SCN1A (Na_V1.1) Antibody (#ASC-001-GP), (1:200).
3,4. Guinea pig Anti-SCN1A (Na_V1.1) Antibody, preincubated with SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001).

Properties

Sequence

(C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774).

Accession (Uniprot) Number P04774

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Storage After Reconstitution -20°C.

Standard Quality Control Of Each Lot Western blot analysis.

Specific Use it with:

Anti-SCN1A (Na_V1.1) Antibody (#ASC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Na_V1.1 from rat, human, and mouse samples.

Anti-SCN1A (Na_V1.1) Antibody (#ASC-001)
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Na_V1.1 channel. Guinea pig Anti-SCN1A (Na_V1.1) Antibody (#ASC-001-GP, formerly AGP-043) raised in guinea pig can be used in western blot analysis. It has been designed to recognize Na_V1.1 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-SCN1A (Na_V1.1) Antibody (#ASC-001) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Guinea pig Anti-SCN1A (Na_V1.1) Antibody (#ASC-001-GP)