Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001) is the original antigen used for immunization during Anti-SCN1A (NaV1.1) Antibody (#ASC-001) generation. The blocking peptide binds and ‘blocks’ Anti-SCN1A/Nav1.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-SCN1A (NaV1.1) Antibody (#ASC-001), (1:200).
2. Anti-SCN1A (NaV1.1) Antibody, preincubated with SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001).
Expression of NaV1.1 sodium channel in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-SCN1A (NaV1.1) Antibody (#ASC-001). A. The distribution of NaV1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of NaV1.1 and Parvalbumin.- Human brain (1:400) (Wang, W. et al. (2011) Brain Res. 1389, 61.).
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) lysates:1,2. Guinea pig Anti-SCN1A (NaV1.1) Antibody (#ASC-001-GP), (1:200).
3,4. Guinea pig Anti-SCN1A (NaV1.1) Antibody, preincubated with SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001).
Properties
Sequence
- (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1 (Accession P04774).
Accession (Uniprot) Number P04774
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
