Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- (C)ASAESRDFSGAGGIGVFSE, corresponding to amino acid residues 467-485 of rat NaV1.2 (Accession P04775).
Accession (Uniprot) Number P04775
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-SCN2A (NaV1.2) Antibody (#ASC-002), (1:200).
2. Anti-SCN2A (NaV1.2) Antibody, preincubated with SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002).- Mouse hippocampus.
Western blot analysis of rat brain (lanes 1 and 3) and mouse brain (lanes 2 and 4) membranes:1,2. Guinea pig Anti-SCN2A (NaV1.2) Antibody (#ASC-002-GP), (1:1000).
3,4. Guinea pig Anti-SCN2A (NaV1.2) Antibody, preincubated with SCN2A/Nav1.2 Blocking Peptide (#BLP-SC002).- Rat paraventricular nucleus (1:2000). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 02/01/2024
