Cat #: BLP-SC007
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900).
Accession (Uniprot) Number P54900
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes under reducing (lanes 1 and 2) or non-reducing (lanes 3 and 4) conditions:*1,3. Anti-SCN2B (NaVβ2) Antibody (#ASC-007), (1:200).
2,4. Anti-SCN2B (NaVβ2) Antibody, preincubated with SCN2B/Navβ2 Blocking Peptide (#BLP-SC007).
* NaVβ2 is disulfide-linked to the α subunit of voltage-gated Na+ channels (Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437.) - Rat and mouse brain sections.
Last update: 02/01/2024