Overview
Type: Synthetic peptide
Form: Lyophilized powder
SCN2B/Navβ2 Blocking Peptide (#BLP-SC007) is the original antigen used for immunization during Anti-SCN2B (NaVβ2) Antibody (#ASC-007) generation. The blocking peptide binds and ‘blocks’ Anti-SCN2B/Navβ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes under reducing (lanes 1 and 2) or non-reducing (lanes 3 and 4) conditions:*1,3. Anti-SCN2B (NaVβ2) Antibody (#ASC-007), (1:200).
2,4. Anti-SCN2B (NaVβ2) Antibody, preincubated with SCN2B/Navβ2 Blocking Peptide (#BLP-SC007).
* NaVβ2 is disulfide-linked to the α subunit of voltage-gated Na+ channels (Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437.)
Expression of NaVβ2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-SCN2B (NaVβ2) Antibody (#ASC-007). A. NaVβ2 channel (red) appears in Purkinje cells (vertical arrows) and in small cells in the molecular layer (horizontal arrows, Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of NaVβ2 to cell bodies. C. Confocal merge of NaVβ2 and CBD28k.
Expression of NaVβ2 in rat cerebellumImmunohistochemical staining of rat cerebellum using Anti-SCN2B (NaVβ2) Antibody (#ASC-007). A. NaVβ2 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of NaVβ2 to cell bodies. C. Confocal merge of NaVβ2 and CBD28k.
Properties
Sequence
- (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels (Accession P54900).
Accession (Uniprot) Number P54900
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
