Name: SCN4B/Navβ4 (extracellular) Blocking Peptide
Brand: Alomone Labs
SKU: BLP-SC044

Overview

Cat #: BLP-SC044

Type: Synthetic peptide

Form: Lyophilized powder

SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044) is the original antigen used for immunization during Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044) generation. The blocking peptide binds and ‘blocks’ Anti-SCN4B/Navβ4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Certificate of Analysis SDS

Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry

For research purposes only. not for human use

Applications

Demonstration of Pre-adsorption control

Western blot analysis of rat brain (lanes 1 and 5), rat cortex (lane 2 and 6), mouse brain (lanes 3 and 7) and SH-SY5Y (lanes 4 and 8) lysates:
1-4. Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044), (1:800).
5-8. Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody, preincubated with SCN4B/Navβ4 (extracellular) Blocking Peptide (#BLP-SC044).
Expression of Na_Vβ4 in rat DRG
Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. A. Na_Vβ4 labeling (red) appears in the cell bodies of the DRG neurons. B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.

Properties

Sequence

(C)KNDKSDPKVRVKDD, corresponding to amino acid residues 85-98 of rat β4 subunit of voltage-gated Na+ channels (Accession Q7M730).

Accession (Uniprot) Number Q7M730

Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.

Purity >70%

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.

Reconstitution 100 µl double distilled water (DDW).

Concentration After Reconstitution 0.4 mg/ml.

Storage After Reconstitution -20°C.

Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.

Standard Quality Control Of Each Lot Western blot analysis.

Specific Use it with:

Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting Na_Vβ4 in living cells. It has been designed to recognize Na_Vβ4 from rat, human, and mouse samples.

Anti-SCN4B (Na_Vβ₄) (extracellular) Antibody (#ASC-044)