Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026) is the original antigen used for immunization during Anti-Secretin Receptor (extracellular) Antibody (#AGR-026) generation. The blocking peptide binds and ‘blocks’ Anti-Secretin Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat pancreas lysate (lanes 3 and 7) and mouse kidney membranes (lanes 4 and 8):1-4. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200).
5-8. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).
Western blot analysis of human PANC-1 pancreatic carcinoma cell lysate:1. Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200).
2. Anti-Secretin Receptor (extracellular) Antibody, preincubated with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026).
Expression of Secretin Receptor in mouse cerebellumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in Bergmann glia profiles (vertical arrows point at Bergmann cell bodies and horizontal arrows at Bergmann glia processes). B. Pre-incubation of the antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).
Expression of Secretin Receptor in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Secretin Receptor (extracellular) Antibody (#AGR-026), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Secretin Receptor immunoreactivity (green) appears in astrocyte profiles (arrows). G = granule layer. OML = outer molecular layer. B. Pre-incubation of the antibody with Secretin Receptor (extracellular) Blocking Peptide (#BLP-GR026), suppresses staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide (C)NSFNERRHAYLLKLK, corresponding to amino acid residues 129 - 143 of rat Secretin Receptor (Accession P23811).
Accession (Uniprot) Number P23811
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
