Overview
Type: Synthetic peptide
Form: Lyophilized powder
Semaphorin 3A/SEMA3A Blocking Peptide (#BLP-SR051) is the original antigen used for immunization during Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin 3A/SEMA3A primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051), (1:400).
3,4. Anti-Semaphorin 3A (SEMA3A) Antibody, preincubated with Semaphorin 3A/SEMA3A Blocking Peptide (#BLP-SR051).
Western blot analysis of human THP-1 acute monocytic leukemia cell lysate (lanes 1 and 3) and human MDA-231 breast adenocarcinoma cell lysate (lanes 2 and 4):1,2. Anti-Semaphorin 3A (SEMA3A) Antibody (#ASR-051), (1:400).
3,4. Anti-Semaphorin 3A (SEMA3A) Antibody, preincubated with Semaphorin 3A/SEMA3A Blocking Peptide (#BLP-SR051).
Properties
Sequence
- (C)KRDRKQRRQRPGH, corresponding to amino acid residues 728-740 of rat SEMA3A (Accession Q63548).
Accession (Uniprot) Number Q63548
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
