Overview
Type: Synthetic peptide
Form: Lyophilized powder
Semaphorin-3F Blocking Peptide (#BLP-SR056) is the original antigen used for immunization during Anti-Semaphorin-3F Antibody (#ASR-056) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin-3F primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes (lanes 1 and 5), rat lung membranes (lanes 2 and 6), mouse heart membranes (lanes 3 and 7) and rat dorsal root ganglion lysate (lanes 4 and 8):1-4. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400).
5-8. Anti-Semaphorin-3F Antibody, preincubated with Semaphorin-3F Blocking Peptide (#BLP-SR056) (#BLP-SR056).
Western blot analysis of human HeLa cervix adenocarcinoma cell line lysate (lanes 1 and 3) and human PC-3 prostate adenocarcinoma cell line lysate (lanes 2 and 4):1, 2. Anti-Semaphorin-3F Antibody (#ASR-056), (1:400).
3, 4. Anti-Semaphorin-3F Antibody, preincubated with Semaphorin-3F Blocking Peptide (#BLP-SR056) (#BLP-SR056).
Properties
Sequence
- (C)RERSAEAPQSPAVYAR, corresponding to amino acid residues 280 - 295 of human Semaphorin-3F (Accession Q13275).
Accession (Uniprot) Number Q13275
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
