Overview
Type: Synthetic peptide
Form: Lyophilized Powder
Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (#BLP-SR065) is the original antigen used for immunization during Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin 4A (SEMA4A) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
Expression of Semaphorin 4A in rat hypothalamusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:300), followed by goat anti-rabbit-Alexa-488. A. Semaphorin 4A immunoreactivity (green) appears in the ventricular wall (horizontal arrows) and in tanycyte processes (vertical arrows). B. Pre-incubation of the antibody with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065), suppressed staining. Cell nuclei are stained with DAPI (blue). 3V = 3rd cerebral ventricle,
VMH = ventromedial hypothalamus.
Properties
Sequence
- Peptide (C)KELNKETSRWTTYRG, corresponding to amino acid residues 355 - 369 of human Semaphorin 4A (Accession Q9H3S1).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
