Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized Powder
Applications: wb, ihc
For research purposes only. not for human use
Properties
Sequence
- Peptide (C)KELNKETSRWTTYRG, corresponding to amino acid residues 355 - 369 of human Semaphorin 4A (Accession Q9H3S1).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
Last update: 02/01/2024
