Overview
Type: Synthetic peptide
Form: Lyophilized powder
Semaphorin 4D/SEMA4D (extracellular) Blocking Peptide (#BLP-SR064) is the original antigen used for immunization during Anti-Semaphorin 4D (SEMA4D) (extracellular) Antibody (#ASR-064) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin 4D/SEMA4D (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody (#ASR-064), (1:200).
3,4. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody, preincubated with Semaphorin 4D/SEMA4D (extracellular) Blocking Peptide (#BLP-SR064).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1,2. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody (#ASR-064), (1:200).
3,4. Anti-Semaphorin-4D (SEMA4D) (extracellular) Antibody, preincubated with Semaphorin 4D/SEMA4D (extracellular) Blocking Peptide (#BLP-SR064).
Properties
Sequence
- (C)EKQHEVYWKVSEDK, corresponding to amino acid residues 80-93 of mouse SEMA4D (Accession O09126).
Accession (Uniprot) Number O09126
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
