Overview
Type: Synthetic peptide
Form: Lyophilized powder
Semaphorin 7A/SEMA7A (extracellular) Blocking Peptide (#BLP-SR077) is the original antigen used for immunization during Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody (#ASR-077) generation. The blocking peptide binds and ‘blocks’ Anti-Semaphorin 7A/SEMA7A (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:1,2. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody (#ASR-077), (1:200).
3,4. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody, preincubated with Semaphorin 7A/SEMA7A (extracellular) Blocking Peptide (#BLP-SR077).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate:1. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody (#ASR-077), (1:400).
2. Anti-Semaphorin 7A (SEMA7A) (extracellular) Antibody, preincubated with Semaphorin 7A/SEMA7A (extracellular) Blocking Peptide (#BLP-SR077).
Properties
Sequence
- (C)RKQEYNGKIPRFRR, corresponding to amino acid residues 190-203 of mouse SEMA7A (Accession Q9QUR8).
Accession (Uniprot) Number Q9QUR8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
