Overview
Type: Synthetic peptide
Form: Lyophilized powder
Septin-7 Blocking Peptide (#BLP-IP047) is the original antigen used for immunization during Anti-Septin-7 Antibody (#AIP-047) generation. The blocking peptide binds and ‘blocks’ Anti-Septin-7 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat spleen (lanes 1 and 4), mouse spleen (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:1-3. Anti-Septin-7 Antibody (#AIP-047), (1:200).
4-6. Anti-Septin-7 Antibody, preincubated with Septin-7 Blocking Peptide (#BLP-IP047) (#BLP-IP047).
Western blot analysis of human Burkitt's lymphoma Daudi (lanes 1 and 3), human T-cell leukemia Jurkat (lanes 2 and 5) and human breast adenocarcinoma MCF-7 cell lysates (lanes 3 and 6):1-3. Anti-Septin-7 Antibody (#AIP-047), (1:200).
4-6. Anti-Septin-7 Antibody, preincubated with Septin-7 Blocking Peptide (#BLP-IP047) (#BLP-IP047).
Properties
Sequence
- (C)HRIKKTVQVEQSK, corresponding to amino acid residues 83-96 of rat Septin-7 (Accession Q9WVC0).
Accession (Uniprot) Number Q9WVC0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
